A. Tosstorff, C. Kroner, D. J. Oppermann, F. Hollmann, D. Holtmann
Old yellow enzymes (OYEs) are able to catalyze asymmetric C = C reductions. A mediated electro enzymatic process to regenerate the NADPH in combination with an OYE was investigated. Due to the fact that the overall process was affected by a broad set of parameters a DoE approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approx. 90% electron transfer efficiency were identified.