Development of a microparticle-enhanced bioprocess for sustainable production of pharmaceutical substances with filamentous microorganisms

Bild Forschungsprojekt
Period: 01.10.2013 - 30.09.2016
Partner: W42 Industrial Biotechnology GmbH, Surflay Nanotech GmbH
Funder: DBU
Project Manager: Dr. Florian Mayer & Ina Huth
Research Group: Biochemical Engineering

Filamentous microorganisms produce a multitude of valuable substances, such as enzymes and secondary metabolites. The industrial production of these substances is often energy intensive, uses a lot of raw materials generates lots of waste. The general goal of the project is to use microparticles to improve the sustainability of processes involving filamentous microorganisms.

In submersed culture filamentous microorganisms develop different morphologies. Growth as free mycelium, as pellets or as an intermediate growth form can be beneficial for product formation, depending on the microbial strain and the product of interest. The addition of microparticles to the culture medium affects the growth form of the organism. Increased control over the morphology simplifies the handling of the process and often improves product formation.

In this project the effects of microparticles on the growth of filamentous microorganisms are investigated. It aims to identify the characteristics of microparticles that are necessary to evoke certain morphologies and simultaneously increase product formation. By developing microparticle-enhanced bioprocesses reaction volumes can be reduced or reaction times decreased, thus minimizing energy consumption, saving raw materials and easing environmental damage.

The DECHEMA Research Institute collaborates with W42 Industrial Biotechnology and Surflay Nanotech. W42 specializes in process and strain development, particularly of filamentous microorganisms, Surflay Nanotech is a leading nanotechnology company.



B.-A. Kaup, K. Ehrich, M. Pescheck, J. Schrader (2008) Microparticle-enhanced cultivation of filamentous microorganisms: Increased chloroperoxidase formation by Caldariomyces fumago as an example. Biotechnology and Bioengineering, 99 (3) 491-498
DOI: 10.1002/bit.21713

R. Walisko, R. Krull, J. Schrader, C. Wittmann (2012) Microparticle based morphology engineering of filamentous microorganisms for industrial bio-production. Biotechnol Lett. 34 (11) 1975-1982
DOI: 10.1007/s10529-012-0997-1

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