Design of aqueous micellar reaction systems for aroma production with carotenoid cleavage dioxygenase.

C. Nacke, M. Schilling, M. M. W. Etschmann, J. Schrader

The use of carotenoid cleavage dioxygenases (CCD) as biocatalysts for aroma generation requires appropriate substrate delivery techniques. Non-ionic surfactants forming micelles represent a versatile tool to introduce hydrophobic carotenoids into aqueous reaction media. In this work, data obtained with CCD1 from Arabidopsis thaliana (AtCCD1) as model enzyme are presented. Previous results from studies with purified carotenoids as model substrates delivered in differently designed micelles are discussed together with new findings on the use of AtCCD1 for carotenoid cleavage out of micellized biomass as a technical substrate. The data presented illustrate the diversified impact of reaction parameters, such as surfactant type, carotenoid structure and source (i.e. purified or as component of biomass) as well as organic (co)solvents, on enzyme activity. New data reveal the possibility to directly cleave carotenoids out of carotenoid containing biomass. For this purpose crude AtCCD1 preparation was added to a homogenate of the carotenoid-rich cyanobacterium Spirulina platensis resulting in specific cleavage and high conversion yield.

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