Pseudomonas putida as universal biocatalyst for the selective oxidation of monoterpenes

Bild Forschungsprojekt
Period:  01.02.2011 - 31.07.2013
Partner:  Universität Düsseldorf, workgroup Urlacher
Funder:  AiF
Research Group:  Biochemical Engineering

The solvent-tolerant bacterium Pseudomonas putida will be optimized by metabolic engineering to meet the challenges of whole-cell biotransformations of hydrophobic and toxic substances such as low biocatalyst activity and stability. The monoterpenes α-pinene and limonene serve as model substances.

The resulting whole-cell biocatalyst represents a platform organism for the expression of cofactor-dependent oxygenases and subsequent use in biotransformations in aqueous-organic two-phase systems. Oxidation of organic compounds is of special interest for the chemical and pharmaceutical industry and this system allows the regio- and stereoselective oxidation beyond the family of monoterpenes. Direct biotechnological access to terpenoids can be established for small- and medium-sized enterprises of the pharmaceutical and fine chemistry industry.

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Das IGF-Vorhaben Nr. der Forschungsvereinigung DECHEMA e.V., Theodor-Heuss-Allee 25, 60486 Frankfurt am Main wurde über die AiF im Rahmen des Programms zur Förderung der industriellen Gemeinschaftsforschung (IGF) vom Bundesministerium für Wirtschaft und Energie aufgrund eines Beschlusses des Deutschen Bundestages gefördert.

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Prof. Dr. Jens Schrader

Tel.: 069/ 7564-337

Dr. Dirk Holtmann

Tel.: 069/ 7564-610

 

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