Caldariomyces fumago as novel expression platform for the production of technical enzymes

Bild Forschungsprojekt
Period:  01.08.2009 - 31.01.2012
Funder:  AiF
Research Group:  Biochemical Engineering

The filamentous fungus C.fumago secretes the versatile protein chloroperoxidase(CPO). This heme containing enzyme exhibits peroxidase, catalase and Cytochrome P450-like activities possessing a broad substrate spectrum. Up to now heterologous overexpression in different expression hosts resulted in inactive enzyme or very low yields. The culture supernatant of C.fumago contains quite high amounts of CPO and nearly no other proteins but a dark undesirable pigment. By colony screening of randomly mutagenized C.fumago cells several white mutants were isolated.  They are still secreting CPO and can therefore be used to produce the enzyme without the contaminating pigment simplifying downstream processing.  Interestingly, CPO activity in the white mutants cultures increase more rapidly compared to wild type cultures.

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Das IGF-Vorhaben Nr. der Forschungsvereinigung DECHEMA e.V., Theodor-Heuss-Allee 25, 60486 Frankfurt am Main wurde über die AiF im Rahmen des Programms zur Förderung der industriellen Gemeinschaftsforschung (IGF) vom Bundesministerium für Wirtschaft und Energie aufgrund eines Beschlusses des Deutschen Bundestages gefördert.

Contact

Prof. Dr. Jens Schrader

Tel.: 069/ 7564-337

Dr. Dirk Holtmann

Tel.: 069/ 7564-610

 

M. Buchhaupt, ... J. Schrader (2011)

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